158 - Liquid chromatography–mass spectrometry analysis to Identify Candidate Biomarkers of Coronary Artery Lesions in Kawasaki Disease

Poster Number: 158
Publication Number: 158.3332

Background: Although several parameters have been reported as candidate biomarkers for predicting the development of coronary artery lesions (CALs) in Kawasaki disease (KD), a reliable marker remains elusive. Liquid chromatography–mass spectrometry (LC/MS)-based proteomic analysis is commonly used for the investigation of biomarkers and next-generation therapeutic targets.

Objective: This study aimed to investigate novel reliable biomarkers for predicting marker of CALs by LC/MS.

Design/Methods: We recruited five patients with and five without CALs among the 299 KD patients admitted to Yamaguchi University Hospital between January 2014 and December 2021. An LC/MS-based proteomic analysis of the sera of KD patients was employed to examine the candidate proteins associated with CALs development.

Results: Six proteins presenting lower ratios in patients with CALs compared to those without were identified. Of the six candidate proteins, histidine-rich glycoprotein (HRG) had the lowest concentration and p-value, and its serum levels were lower in patients with CALs than in those without CALs by enzyme-linked immuno-sorbent assay.

Conclusion(s): The results of the present study suggested HRG might be a potential biomarker for predicting the development of CALs and therapeutic target in KD.